Isolation of Vaccine-Like Poliovirus Strains in Sewage Samples From the United Kingdom

Journal of Infectious Diseases
Volume 217, Issue 8  15 April 2018
https://academic.oup.com/jid/issue

MAJOR ARTICLES AND BRIEF REPORTS
Isolation of Vaccine-Like Poliovirus Strains in Sewage Samples From the United Kingdom
Manasi Majumdar; Dimitra Klapsa; Thomas Wilton; Joyce Akello; Catherine Anscombe
The Journal of Infectious Diseases, Volume 217, Issue 8, 28 March 2018, Pages 1222–1230, https://doi.org/10.1093/infdis/jix667
We describe the isolation of vaccine-like poliovirus in sewage samples using concentration methods followed by cell culture infection and next generation sequencing. Using this approach, we rapidly obtained whole-genome sequences of polio and nonpoliovirus enterovirus strains present in mixtures.