Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential application in epidemiological research & bio banking

Gates Open Research
https://gatesopenresearch.org/browse/articles
[Accessed 16 Nov 2019]

 

Research Article
Revised
Optimization of extraction of genomic DNA from archived dried blood spot (DBS): potential application in epidemiological research & bio banking [version 3; peer review: 2 approved with reservations]
Abhinendra Kumar, Sharayu Mhatre, Sheela Godbole, Prabhat Jha, Rajesh Dikshit
Limited infrastructure is available to collect, store and transport venous blood in field epidemiological studies. Dried blood spot (DBS) is a robust potential alternative sample source for epidemiological studies & bio banking. A stable source of genomic DNA (gDNA) is required for long term storage in bio bank for its downstream applications. Our objective is to optimize the methods of gDNA extraction from stored DBS and with the aim of revealing its utility in large scale epidemiological studies.